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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene continues to be observed in many tumors, which ends up in chromosome instability, and so may add to tumorigenesis. This protein was found to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a significant role in promoting cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are described. Two supplemental splice variants had been claimed but specific nucleotide sequence data will not be nonetheless offered.

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Blocking peptides are peptides that bind especially on the goal antibody and block antibody binding. These peptide normally includes the epitope acknowledged via the antibody.

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This manual outlines even further probable optimization of cellular and immunoassay parameters to be sure the absolute best success are...

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This guideline outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best effects are...

Antibodies certain to the blocking peptide no thr77 more bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody vs . the antibody by itself, you can see which staining is distinct.

Goal Details Cyclin E belongs for the remarkably conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show distinct expression and degradation designs which lead to the temporal coordination of every mitotic celebration. Cyclin E kinds a fancy with and functions like a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is particularly degraded as cells progress by S section.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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